Comparison of transport media for the isolation and detection of Brachyspira hyodysenteriae

Cho, Se-Ji; Kim, Jong Wan; Kim, Ha-Young; Oh, Sang-Ik; Jeong, So Jeong; Jung, Ji-A; Cho, Ara; Lee, Myoung-Heon; Cho, Ho-Seong; Byun, Jae-Won

doi:2015.55.1.9

Korean J Vet Res > Volume 55(1); 2015 > Article

Original Article

Korean Journal of Veterinary Research 2015;55(1):9-12.
DOI: https://doi.org/10.14405/kjvr.2015.55.1.9 Published online March 24, 2015.

Comparison of transport media for the isolation and detection of Brachyspira hyodysenteriae

Se-Ji Cho¹, Jong Wan Kim¹, Ha-Young Kim¹, Sang-Ik Oh¹, So Jeong Jeong¹, Ji-A Jung¹, Ara Cho¹, Myoung-Heon Lee¹, Ho-Seong Cho², Jae-Won Byun¹

¹Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency
²College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University

돈적리 균의 분리, 검출을 위한 수송배지의 비교

조세지¹, 김종완¹, 김하영¹, 오상익¹, 정소정¹, 정지아¹, 조아라¹, 이명헌¹, 조호성², 변재원¹

¹농림축산검역본부
²전북대학교 수의과대학

Abstract

Brachyspira (B.) hyodysenteriae is a causative agent of swine dysentery that is responsible for death and economic losses in the pig industry. It is imperative that clinical samples be delivered fresh for accurate diagnosis. The viability and DNA detection of B. hyodysenteriae using lab-made (phosphate buffered saline and modified tryptic soy broth) or commercial transport media (C, D, and E) were compared by culturing and real-time PCR at $4^{circ}C$ or room temperature (RT), respectively. B. hyodysenteriae grown in D (Anaerobe Systems, USA) and E (Starplex Scientific, Canada) media was viable for 4 days at $4^{circ}C$ and RT. However, B. hyodysenteriae in A, B, and C (culture swab; BD Biosciences, USA) media were not recovered after 2 days at RT. Ct values for real-time PCR at $4^{circ}C$ and RT ranged from $27.2{pm}2.1$ (C) to $29.6{pm}0.5$ (B), and $28.0{pm}0.9$ (E) to $30.2{pm}1.5$ (B), respectively. Considering the field conditions, it is important that transport media is used for specimen isolation and PCR to obtain an accurate diagnosis of swine dysentery.

Key Words: Brachyspira hyodysenteriae, pigs, real-time PCR, transport media