Changes of plasma progesterone concentrations after induction of estrus in the bitch

Kang, Byong-kyu; Choi, Han-sun; Na, Jin-su; Lee, Cha-bum; Oh, Ki-seok; Son, Chang-ho

Korean Journal of Veterinary Research 1989;29(3):253-262.

Byong-kyu Kang¹, Han-sun Choi¹, Jin-su Na², Cha-bum Lee¹, Ki-seok Oh¹, Chang-ho Son¹

¹College of Veterinary Medicine, Chonnam National University
²Department of Animal Science, College of Agriculture, Chonnam National University

발정유기(發情誘起)된 암캐의 혈중(血中) progesterone 농도변화(濃度變化)

강병규¹, 최한선¹, 나진수², 이차범¹, 오기석¹, 손창호¹

¹전남대학교 수의과대학
²전남대학교 농과대학 축산학과

Abstract

This study was performed to investigate the patterns of progesterone secretion after induction of estrus in premature, metestrous and anestrous bitches. A total of 22 bitches were used. Of them 18 bitches were treated with hormone to induced estrus and 4 bitches were untreated and served as controls. Estrus was induced with $PGF_{2{alpha}}$, estrone, estradiol-$17{eta}$, PMSG and HCG(Treatment A), and with PMSG and HCG(Treatment B). Blood samples were collected via the cephalic vein at 2 to 5 days interval. Blood samples were centrifuged (1,200g, 10min.) within 30 minutes after collection and plasma was stored at $-20^{circ}C$ until analyzed for the progesterone concentrations. Plamsa progesterone concentrations were measured by radioimmunoassay. The results of estrous induction were determined by estrous signs, ovarian response, egg recovery and progesterone patterns. The results obtained were as follows; 1. All bitches in treatment A showed estrous signs, however the ovarian response and egg recovery were not detectable and the levels of progesterone were nearly same as before. 2. In the treatment B, premature and metestrous bitches showed only estrous signs, however 5 of 7 anestrous bitches (71.4%) showed estrous signs, ovarian response and changes of progesterone levels. In conclusion, clinical estrous behavior can be induced during any phase of the estrous cycle, but ovulation should be induced only if induction occur approximately 4 months or more after the previous estrus.

Key Words: bitches, progesterone patterns, estrous induction, radioimmunoassay, ovulation