Immunohistochemical identification of newcastle disease virus with indirect immunoperoxidase technique

Nho, Whan-goog; Sur, Jung-hyang; Kim, Soon-bok

Korean Journal of Veterinary Research 1990;30(3):309-315.

Whan-goog Nho, Jung-hyang Sur, Soon-bok Kim

College of Veterinary Medicine, Gyeongsang National University

Indirect Immunoperoxidase 법을 이용한 조직내 뉴켓슬병 바이러스 항원동정

노환국, 서정향, 김순복

경상대학교 수의과대학

Abstract

The present experiment was done to identify newcastle disease virus(NDV) antigens in frozen sections of various oragns from experimentally NDV-infected with indirect immunoperoxidase method. Section were incubated with rabbit anti-NDV polyclonal as first antibody, followed by incubation with goat anti-rabbit or protein A peroxidase conjugate. Positive reactions were often detected in the epithelium of trachea and in the lymphocyte of spleen at 24 hours after virus inoculation. the viral antigen was localized mainly in the cytoplasm of infected cells. The method approved to be highly specific for the identification of NDV and allowed a precise localization of the viral antigens in infected cells.

Key Words: Newcastle disease virus, indirect immunoperoxidase technique, chicken