Effect of renal ischemia on amino acid transport in rabbit renal cortical slices

Nam, Yun-jeong; Kim, Joo-heon

Korean Journal of Veterinary Research 1997;37(1):111-117.

Yun-jeong Nam, Joo-heon Kim

College of Veterinary Medicine and Institute of Animal Medicine Gyeongsang National University

신장 허혈이 토끼 신피질 절편에서 아미노산 이동에 미치는 영향

남윤정, 김주헌

경상대학교 수의과대학, 동물의학연구소

Abstract

This study was carried out determine the effect of renal ischemia on amino acid transport in rabbit renal cortical slices. The animal models of renal ischemia induced experimentally by clamping the renal artery. These results were summarized as follows: 1. The uptake of amino acids lysine and ${alpha}$-aminobutyrate(AIB), dicarboxylate succinate and organic anion PAH in cortical slices was normal or increased after 30 or 60 min of ischemia in vivo. 2. In a 30 min ischemic kidney, the slice uptake of amino acids was returned to the control level by 30 min of reflow. In a 60 or 90 min ischemic kidney, the lysine uptake was returned to the control level after of reflow, but the uptake of AIB and succinate was significantly reduced during reflow period of 30-120 min. 3. Oxygen consumption in cortical slices was increased after 30 min of ischemia but was not altered by 60 min of ischemia. This results indicat that transient ischemia caused increasing of amino acid uptake in renal cortical slices without metabolic disorder of renal proximal tubule.

Key Words: amino acid, renal ischemia, renal cortical slice, oxygen consumption