Immunohistochemical electron microscopic studies on somatotropes and mammotropes in hypophysis of Korean native goat

Lee, In-se; Lee, Heungshik S.; Won, Moo-ho; Seo, Jehoon; Song, Seung-hoon; Nam, Young-Sam; Kang, Tae-Cheon

Korean Journal of Veterinary Research 1998;38(3):488-496.

In-se Lee¹, Heungshik S. Lee¹, Moo-ho Won², Jehoon Seo¹, Seung-hoon Song¹, Young-Sam Nam¹, Tae-Cheon Kang³

¹College of Veterinary Medicine, Seoul National University
²College of Medicine, Seoul National University
³College of Medicine, Hallym University

한국재래산양 뇌하수체의 성장자극세포와 젖샘자극세포에 관한 전자현미경적 연구

이인세¹, 이흥식¹, 원무호², 서제훈¹, 송승훈¹, 남영삼¹, 강태천³

¹서울대학교 수의과대학
²서울대학교 의과대학
³한림대학교 의과대학

Abstract

Somatotropes, mammotropes and somatomammotropes of the Korean native goat hypophysis were studied by double immunoelectron microscopy using antisera to growth hormone(GH) and prolactin(PRL), and protein A-gold particles of different sizes. Mammotropes were round or oval in shape, and contained round and electron dense secretory granules. The size of secretory granules was variable from 460nm to 680nm in diameter. Somatotropes were elliptical or triangular in shape and the oval nucei were located eccentrically at the periphery of the cell. Secretory granules of the cell were oval in shape and clearly distinguished from round granules of mammotropes. The size of granules was 320~680nm in diameter, smaller than that of mammotropes. Somatomammotropes contained round or oval secretory granules. The granules had intermediate size between somatotropes and mammotropes. Some of granules contained both GH and PRL, while the others contained only one of them.

Key Words: mammotropes, somatotropes, somatomammotropes, double immunoelectron microscopy, Korean native goat