| Control of swine respiratory disease using egg yolk antibodies II. Specificity of immunoglobulin Y of hens immunized with bacterial pathogens related with swine respiratory diseases |
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Na-ri Shin1, Jong-man Kim2, In-soo Choi1, Han sang Yoo1 |
1Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University
2Division of Bacteriology and Immunology, National Veterinary Research and Quarantine Service |
| 난황항체를 이용한 돼지 호흡기 질병 방제에 관한 연구 II. 면역된 산란계로부터 생산된 난황항체의 특이성 분석 |
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신나리1, 김종만2, 최인수1, 유한상1 |
1서울대학교 수의과대학 및 농생명공학부
2국립수의과학검역원 세균과 |
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| Abstract |
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Although several methods have been developed and applied to control swine respiratory diseases, the disease induces severe economic impact to swine industry worldwide. As one of the new trials, application of egg yolk antibody(IgY) was attempted for the purposes and immune response in sera and egg yolk was analysed with ELISA in previous study. In this study, immunological specificity of the IgY was analysed by Western blot analysis. In the analysis of causative agents of atrophic rhinits, B bronchiseptica and P multocida 4D, proteins of 33, 40, 43, 67 and 141 kDa were specifically reacted with IgY Also, 40 and 110 kDa proteins were identified as the major immunogens in P multocida 3A. In A pleuropneumoniae serotypes 2 and 5, 40 kDa and 47 kDa proteins were found to be the major reactive ones. These results suggested that egg yolk antibodies from immunized hens was specific with antigens injected into hens and partially purified antigens, outer membrane proteins and dermonecrotic toxin, were more effective than bacterin for the production of specific antibody. |
| Key Words:
IgY, specificity, Western blot, swine, respiratory diseases |
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