| Recovery and Survival of Listeria monocytogenes in Surface and Sea Water |
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Ju Yang1, Toh-Gyong Kim2, Ho-Jo Kang1 |
1College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University
2Kyongnam Livestock Promotion Institute |
| 지표수 및 해수로부터 Listeria monocytogenes의 분리 및 생존성 |
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양주1, 김도경2, 강호조1 |
1경상대학교 수의과대학/동물의학연구소
2경상남도 축산진흥연구소 |
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| Abstract |
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The study was carried out to examine the distribution and survival rate of Listeria monocytogenes (L monocytogenes) from various source of waters using improved isolation method. In comparision of enrichment media for isolation of L monocytogenes from water, the isolation rate and 50% detection limit of the pathogen were higher in UVM modified Listeria enrichment broth (UVM) than Listeria enrichment broth (LEB). On the other hand, when compared the selective media for isolation of the pathogen from water, the isolation rate was highest in culture at Oxford agar followed by Fraser agar, and LEB agar. In order to improve enrichment method, 100 ml of water samples with 0.1 CFU/ml of L monocytogenes was inoculated into 10 ml of UVM concentrated at 10-fold, and incubated for 24 h at $36^{circ}C$. Isolated frequency of the pathogens in improved enrichment method completely corresponded with common (filter) method. Of a total mumber of 147 water samples from river, lake and sea, the pathogen was isolated from 1 of 39 (2.6%) river water samples and 1 of 75 (1.3%) sea water samples, but no pathogen was isolated from 33 lake water samples. Serotypes of 2 isolates were identified as type 1. L monocytogenes decreased in number from 7.2-7.4 to 4.2-4.7 log CFU/ml for 1 week poststorage (5 and $20^{circ}C$), but the pathogens were able to be detected in river and sea water until 8 weeks after storage. However, in tap water, L monocytogenes were decreased to undetectable level after 2 weeks of storage. |
| Key Words:
Listeria monocytogenes, isolation, survival, surface water, sea water |
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