| Immunologic effects of somatic antigens of house dust mite (Dermatophagoides pteronyssinus) against canine sarcoptic mite (Sarcoptes scabiei var. canis) infestation |
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In-Soo Yoon, Jae-Won Kim, Cha-Ho Jee |
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College of Veterinary Medicine and Research Institute of Veterinary Medicine Chungbuk National University |
| 집먼지진드기 체항원을 이용한 개 옴 감염증에 대한 면역효과 |
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윤인수, 김재원, 지차호 |
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충북대학교 수의과대학 및 동물의학연구소 |
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| Abstract |
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Canine sarcoptic mite (Sarcoptes scabiei var. canis) burrow usually in the stratum corneum of the skin of dogs and rabbits. Antigens from the burrowing mites induce cutaneous inflammatory reaction and humoral and cell-mediated immune response in the host. The effect of immunization induced by somatic antigens of house dust mite (Dermatophagoides spp.) has been evaluated to control the canine sarcoptic mite in this experiment. Twelve common antigens (187, 142, 126, 120, 109, 92, 80, 68, 51, 30, 25, 17 kDa) were found using SDS-PAGE with silver staining and Western blot between canine sarcoptic mite and house dust mite. In order to evaluate the immunologic effect of these common antigens 10 New Zealand white rabbits were divided as 4 groups such as negative control (group I), positive challenged control (group II), vaccinated (group III), and vaccinated-challenged (group IV) groups. Group II was artificially infested with about 1,000 canine sarcoptic mites and group III and IV were immunized with somatic antigens of house dust mite. In addition group IV was artificially infested with about 1,000 canine sarcoptic mites and group II, IV were treated with ivermectin. At the 8 weeks of the vaccination with common antigen, the antibody titers of all groups of II, III and IV had been increased. Both infestation score and live canine sarcoptic mite counts of group IV were lower than group III. Infestation score of group II become 0 by 2 weeks and group IV by 4 weeks after infestation. These results suggest that house dust mite, which is easy to culture in vitro, can be a vaccine candidate for protection of canine sarcoptic mite infestation. |
| Key Words:
Canine sarcoptic mite (Sarcoptes scabiei var. canis), house dust mite (Dermatophagoides spp.), somatic antigens, infestation score, vaccination |
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