| Genetic characterization of porcine circovirus 2 Korean isolates |
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Choi-Gyu Park1, Kyoung-Ki Lee1, Hyun-Soo Kim2 |
1National Veterinary Research and Quarantine Services
2College of Veterinary Medicine, Chungnam National University |
| Porcine circovirus 2 국내 분리주의 유전적 특성 |
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박최규1, 이경기1, 김현수2 |
1국립수의과학검역원
2충남대학교 수의과대학 |
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| Abstract |
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In order to obtain the genetic informations of the Korean isolates of porcine circovirus 2 (PCV2), nucleotide sequences of total genome of three isolates and open reading frame 2 (ORF2) of four isolates were determined and compared with those of other reference PCV2 isolates. Nucleotide sequences of 3 isolates showed over 99% homology with those of reference strain (GenBank accession no. AF027217). Point mutations were mainly determined on ORF2 regions but little on ORF1 regions. The patterns of pointmutated sites and nucleotide substitution on ORF2 regions were generally consistent between Korean isolates, and these mutated sites observed in Korean isolates were also relatively similar to those of foreign isolates. Phylogenetic analysis of nucleotide or amino acid sequences showed that there were minor branches consisting of three clusters; cluster of Korea, Canada and America, cluster of Spain and Taiwan, and the last cluster of French and China isolates. These results suggested that Korean PCV2s were probably originated from North America such as Canada or USA. The genetic informations obtained from this study could be useful for the research of diagnosis and pathogenecity of PCV2. |
| Key Words:
postweaning multisystemic wasting syndrom(PMWS), porcine circovirus(PCV), nucleotide sequence, open reading frame(ORF), phylogenetic tree |
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