Functional characterization of <TEX>$P_{2X}/P_{2Y}$</TEX> receptor in isolated swine renal artery

Kim, Joo-heon; Jeon, Je-cheol; Lee, Sang-kil; Lee, Su-jin; Lee, Younggeon; Won, Jinyoung; Kang, Jae seon; Hong, Yonggeun

Korean Journal of Veterinary Research 2007;47(4):371-378.

Functional characterization of $P_{2X}/P_{2Y}$ receptor in isolated swine renal artery

Joo-heon Kim¹, Je-cheol Jeon¹, Sang-kil Lee¹, Su-jin Lee², Younggeon Lee², Jinyoung Won², Jae seon Kang³, Yonggeun Hong²

¹College of Veterinary Medicine, Gyeongsang National University
²College of Biomedical Science & Engineering, Inje University
³College of Engineering, Inje University

Abstract

To understand the role of $PM_{2X}/P_{2Y}$ receptor in cortex region of kidney and renal artery, molecular and functional analysis of $PM_{2X}/P_{2Y}$ receptor by pharmacophysiological skill in conventional swine tissues were performed. In functional analysis of $P_{2Y}$ receptor for vascular relaxation, 2-methylthio adenosine triphosphate, a strong agonist of $P_{2Y}$ receptor, induced relaxation of noradrenaline (NA)-precontracted renal artery in a dose-dependent manner. Strikingly, relaxative effect of ATP, 2-msATP, agonists of $P_{2Y}$ receptor, abolished by treatment of reactive blue 2, a putative $P_{2Y}$ receptor antagonist. In contrast, no significant differences of gene encoding $PM_{2X}/P_{2Y}$ and protein expression in immortalized suprachiasmatic nucleus from brain, primary isolated vascular smooth muscle cells from renal artery of pigs and HEK293 from human embryonic kidney under with/without adenosine triphosphate were observed. Taken together, the relationship between molecular and functional characteristic of $PM_{2X}/P_{2Y}$ receptors in conventional pig should be considered that they are another important factor which regulate the kidney function in swine. Based on this study, we propose the purinergic receptor as well as adrenergic and cholinergic receptors is an essential component of the renal homeostasis.

Key Words: adenosine triphosphate, 2-methylthio adenosine triphosphate, $PM_{2X}/P_{2Y}$ receptor, reactive blue 2, swine renal artery