Pre- and postnatal development study of amitraz in rats

Kim, Sung-Hwan; Lim, Jeong-Hyeon; Park, Na-Hyeong; Moon, Changjong; Park, Soo-Hyun; Kang, Seong-Soo; Bae, Chun-Sik; Kim, Sung-Ho; Shin, Dong-Ho; Kim, Jong-Choon

Korean Journal of Veterinary Research 2010;50(2):93-103.

Sung-Hwan Kim, Jeong-Hyeon Lim, Na-Hyeong Park, Changjong Moon, Soo-Hyun Park, Seong-Soo Kang, Chun-Sik Bae, Sung-Ho Kim, Dong-Ho Shin, Jong-Choon Kim

College of Veterinary Medicine, Chonnam National University

랫드에서 amitraz의 출생 전후 발생 시험

김성환, 임정현, 박나형, 문창종, 박수현, 강성수, 배춘식, 김성호, 신동호, 김종춘

전남대학교 수의과대학

Abstract

This study investigated the potential effects of amitraz on the pre- and postnatal development, behavior, and reproductive performance of offspring of parent rats given amitraz during pre-mating, gestation, and lactation. The test chemical was administered via the drinking water containing 0, 40, 120, and 360 ppm to male rats from 2 weeks before mating to the end of 14-day mating period and to females from 2 weeks before mating, throughout mating, gestation and lactation up to weaning. Based on fluid consumption, the male rats received an average of $0,;5.7{pm}1.33,;13.2{pm}2.08,$ and $35.8{pm}3.42$ mg/kg/day amitraz, and the female rats received an average of $0,8.7{pm}4.42,;20.1{pm}9.60,;and;47.6{pm}22.38$ mg/kg/day amitraz, respectively. At 360 ppm, an increase in the incidence of abnormal clinical signs, a suppression in the body weight gain, a decrease in the food consumption and litter size, an increase in the post-implantation loss, and a decrease in the seminal vesicle weight were observed in the parent animals. In addition, a suppression in the body weight gain, a decrease in the grip strength, a delay in the negative geotaxis, an increase in the pre- and post-implantation loss, and a decrease in the number of live embryos were observed in the offspring. At 120 ppm, suppressed body weight gain and reduced food consumption were observed in the parent rats. Suppressed body weight gain and decreased grip strength were also observed in the offspring. There were no signs of either reproductive or developmental toxicity at 40 ppm. Under these experimental conditions, the no-observed-adverse-effect level of amitraz for parent rats and their offspring was estimated to be 40 ppm in rats.

Key Words: amitraz, pre- and postanatal study, rats, reproductive and developmental toxicity