| Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection |
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Kiju Kim1, Dooree Kim1, Jisun Sun1, Soyeon Park1, Youngjae Cho1, Hyun-Jeong Ko2, Hong-Gu Joo3, Tae-Wook Hahn1 |
1College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University
2College of Pharmacy, Kangwon National University
3College of Veterinary Medicine, Jeju National University |
| Salmonella enterica serovars Enteritidis, Gallinarum 및 Typhimurium의 마우스 면역반응의 비교평가 |
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김기주1, 김두리1, 선지선1, 박소연1, 조영재1, 고현정2, 주홍구3, 한태욱1 |
1강원대학교 수의과대학 및 동물의학종합연구소
2강원대학교 약학대학
3제주대학교 수의과대학 |
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| Abstract |
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The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine. |
| Key Words:
humoral immunity, mucosal immunity, Salmonella |
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