| Temporal and subcellular distributions of Cy5.5-labeled hyaluronic acid nanoparticles in mouse organs during 28 days as a drug carrier |
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Chunmei Lin1, Saet Byeol Kim2, Jung-Min Yon2, Seul Gi Park2, Lee Wha Gwon2, Jong-Geol Lee2, In-Jeoung Baek3, Beom Jun Lee2, Young Won Yun2, Sang-Yoon Nam2 |
1College of Chinese Medicinal Materials, Jilin Agricultural University
2College of Veterinary Medicine and Veterinary Medical Center, Chungbuk National University
3Asan Institute for Life Sciences, Asan Medical Center and University of Ulsan |
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| Abstract |
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Temporal and subcellular distributions of hyaluronic acid (HA) as a degradable nanoparticle (NP) in animals were investigated to determine if HA-NP could be utilized as an appropriate drug delivery system. After mice were intravenously injected with 5 mg/kg of Cy5.5-labeled HA-NP sized 350-400 nm or larger HA-polymers, the fluorescence intensity was measured in all homogenized organs from 0.5 h to 28 days. HA-NP was greatly detected in spleen, liver and kidney until day 28, while it was maintained at low levels in other organs. HA-polymer was observed at low levels in all organs. HA-NP quantities in spleen and liver were reduced until day 3, but increased sharply between days 3 and 7, then decreased again, while their HA-polymers were maintained at low levels until day 28. In kidneys, both HA-NP and HA-polymer showed high levels after 0.5 h of administration, but steadily decreased until day 28. According to ultrastructural analyses, HA-NP was engulfed in Kupffer cells of liver and macrophages of spleen and kidney at day 1 and was accumulated in the cytoplasm of kidney tubular cells at day 7. Overall, these findings suggest that HA-NP could be considered a desirable drug carrier in the liver, kidney, or spleen. |
| Key Words:
drug delivery systems, hyaluronic acid, nanoparticles, pharmacokinetics, subcellular localization |
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