| Tumorsphere formation and cancer stem cell characterization of REM134 canine mammary carcinoma cells |
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Jeong Su Byeon, Jienny Lee, Da-Un Jeong, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha |
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Viral Disease Research Division, Animal and Plant Quarantine Agency |
| 개 REM134 유선종양세포의 sphere 형성을 통한 암 줄기세포 특성 분석 |
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변정수, 이지현, 정다운, 구나연, 조인수, 차상호 |
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농림축산검역본부 바이러스질병과 |
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| Abstract |
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Canine mammary tumors are among the most frequently observed cutaneous tumors in female dogs. Cancer stem cells (CSCs), referred to as tumor-initiating cells, are thought to have properties similar to normal stem cells such as the ability to self-renewal and to differentiate into various cell types. Biological understanding of CSCs and the critical pathways involved in their maintenance are important in research and therapy for mammary tumors. We conducted the present study on sphere formation from REM134 cells by using methylcellulose to produce tumorspheres on a large scale and compared the specific markers of the spheres-formed and plating-cultured REM134 cells. The results revealed that the tumorspheres cultured in methylcellulose had higher seeding density and improved morphology compared to those produced in normal sphere formation medium. Expression levels of stemness markers and CSC-related markers were higher in tumorsphere-forming cells than in plating-cultured cells. Subsequently, we transplanted the tumorsphere-forming and plating-cultured cells into female nude mice to examine their tumorigenic potential. Tumor volume increased rapidly in mice transplanted with tumorsphere-derived cells compared to plating-cultured cells. We observed a novel sphere-forming condition for REM134 cells and showed that REM134 cell tumorspheres can exhibit improved CSC properties. |
| Key Words:
animal mammary neoplasms, carcinogenesis, neoplastic stem cells |
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