Modulatory action of enrofloxacin in lipopolysaccharide-induced hyper-activated mouse spleen cells

Ko, Hyeong-Ju; Joo, Hong-Gu

doi:2019.59.2.75

Korean J Vet Res > Volume 59(2); 2019 > Article

Original Article

Korean Journal of Veterinary Research 2019;59(2):75-80.
DOI: https://doi.org/10.14405/kjvr.2019.59.2.75 Published online July 15, 2019.

Modulatory action of enrofloxacin in lipopolysaccharide-induced hyper-activated mouse spleen cells

Hyeong-Ju Ko, Hong-Gu Joo

College of Veterinary Medicine, Veterinary Medical Research Institute, Jeju National University

Lipopolysaccharide에 의해 과활성화된 마우스 비장세포에서 Enrofloxacin의 조절작용

고형주, 주홍구

제주대학교 수의과대학, 수의과학연구소

Correspondence: Hong-Gu Joo, Tel: 82-64-754-3379, Fax: 82-64-756-3354,
Email: jooh@jejunu.ac.kr

Abstract

Enrofloxacin, a fluoroquinolone, is a broad-spectrum antibiotic widely used in veterinary medicine that inhibits the action of bacterial DNA gyrase, resulting in anti-bacterial effects. This study was performed to examine whether enrofloxacin has modulatory and anti-inflammatory activity on immune cells. A few studies have reported the anti-inflammatory effects of enrofloxacin. In this study, we used mouse spleen cells treated with lipopolysaccharide (LPS) and examined the effects of enrofloxacin. Several assays were performed in LPS-treated spleen cells after the enrofloxacin treatment. Enrofloxacin inhibited the metabolic activity and mitochondrial membrane potential of LPS-treated spleen cells significantly. On the other hand, enrofloxacin did not alter the proportion of the subsets in spleen cells, and did not induce cell death. The production of tumor necrosis factor-alpha in LPS-treated spleen cells was inhibited by enrofloxacin. Overall, enrofloxacin had modulatory activity in spleen cells treated with LPS. These data may broaden the use of enrofloxacin as an antibiotic with anti-inflammatory activity in veterinary clinics.

Key Words: enrofloxacin, spleen cells, anti-inflammatory activity, lipopolysaccharide, modulatory action