Enrichment of Salmonella choleraesuis in Tetrathionate Broth -Effect of Concentration of Iodine Solution on the Growth of Sal. choleraesuis-

Chung, Gill Taik; Tak, Ryun Bin

Korean Journal of Veterinary Research 1976;16(1):53-57.

Gill Taik Chung¹, Ryun Bin Tak²

¹College of Veterinary Medicine, Seoul National University
²Department of Veterinary Medicine, College of Agriculture, Gyeongbug National University

Salmonella choleraesuis의 증균(增菌)에 관한 연구 -Tetrathionate Broth Base에 대한 옥도용액(沃度溶液)의 함량(含量)이 증균효과(增菌效果)에 미치는 영향-

정길택¹, 탁연빈²

¹서울대학교 수의과대학
²경북대학교 농과대학 수의학과

Abstract

The growth of Sal. choleraesuis and its kunzendorf variety in tetrathionate broth containing various amounts of iodine solution was studied and compared with that of Sal. typhi, Sal. typhimurium and E. coli. The results obtained were as followings. 1. When 2.0 ml of iodine solution, normal amount, was added to 100 ml of tetrathionate broth base, the number of Sal. choleraesuis decreased rapidly until 24 hours after inoculation and slightly increased 48 hours after inoculation. The numbers of Sal. choleraesuis var. kunzendorf and E. coli decreased rapidly and none of the organisms recovered 24 and 48 hours after inoculation, respectively. The growth of Sal. typhi and Sal. typhimurium, however, was not inhibited at all. 2. When 4.0 ml of iodine solution was added, to 100 ml of tetrathionate broth base, the growth of all the organisms was inhibited, among which Sal. choleraesuis, Sal. choleraesuis var. kunzendorf, and E. coli were not recovered 24 and 48 hours after inoculation. 3. When reduced amounts of iodine solution, 1.0 ml and 0.5 ml, were added to 100 ml of tetrathionate broth base, the growth of all the organisms was not inhibited.