| Immunohistochemistry for detection of Aujeszky's disease virus antigens : Immunogold-silver method in tissue sections |
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Soon-bok Kim |
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Department of Veterinary Medicine, Gyeongsang National University |
| 오제스키병 바이러스 항원검출을 위한 면역조직화학적 연구 -조직절편내 immunogold-silver법- |
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김순복 |
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경상대학교 수의학과 |
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| Abstract |
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The present study was done to demonstrate ADV antigens in frozen and paraffin sections from ADV-infected pigs and cell cultures by using of the IGS method. Tissue specimens from 3 young pigs infected with ADV-phylaxia strain and of 2 healthy pigs were used. Fibroblastic cells originated from pig brain and BHK cells were grown and confluent monolayers were infected with the virus. Two monoclonal antibodies and a specific hyperimmune serum to ADV were used as the source of primary antibodies for both the IGS and immunoperoxidase methods. Application of the IGS method yielded a black fine granular reaction in positive areas, and the results were superior to those obtained using the immunoperoxidase technique for all cases tested. The IGS method might be useful in the detection of various viral antigens in tissue sections. |
| Key Words:
immunogold-silver method, Aujeszky's disease virus antigens |
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