| Protective effect of ethyl acetate extract of Ishige okamurae against carbon tetrachloride-induced acute liver injury in rats |
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Sohi Kang1, Wonjun Yang1, Hanseul Oh1, Yeonji Bae1, Meejung Ahn2, Min Chul Kang3, Ryeo Kyeong Ko3, Gi Ok Kim3, Jun Hwa Lee4, Jin Won Hyun5, Changjong Moon6, Taekyun Shin1 |
1Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University
2Department of Anatomy, College of Medicine, Jeju National University
3Jeju Technopark
4Department of Pediatrics, Samsung Changwon Hospital, School of Medicine, Sungkyunkwan University
5Department of Biochemistry, College of Medicine, Jeju National University
6Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University |
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| Abstract |
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Several compounds and extracts isolated from a brown alga, Ishige (I.) okamurae, exhibit anti-oxidant and anti-inflammatory effects. The present study investigated whether the ethyl acetate (EtOAc) fraction of I. okamurae (EFIO) could ameliorate carbon tetrachloride ($CCl_{4}$)-induced hepatotoxicity in rats. Sprague-Dawley rats were intraperitoneally (i.p.) administered with EFIO at 10 or 50 mg/kg per day for 2 consecutive days before $CCl_{4}$ injection (3.3 mL/kg, i.p.). Twenty four hours later, the rats were anesthesized with diethyl ether and dissected. Pretreatment with EFIO significantly reduced the increased serum levels of alanine aminotransferase and aspartate aminotransferase in $CCl_{4}$-treated rats. Pretreatment with EFIO also significantly inhibited the reduced activities of superoxide dismutase and catalase in the $CCl_{4}$-injured liver. Histopathological evaluations showed that hemorrhage, hepatocyte necrosis, inflammatory cell infiltration, and fatty degeneration induced by $CCl_{4}$ treatment were ameliorated by the administration of EFIO. Additionally, liver immunohistochemical analyses revealed the marked reduction in ED1-positive monocyte-like macrophages in EFIO-pretreated rats given $CCl_{4}$. These results suggest that EFIO ameliorates $CCl_{4}$-induced liver injury, possibly through the inhibition of oxidative stress. |
| Key Words:
antioxidant, carbon tetrachloride, hepatoprotection, Ishige okamurae, serum chemistry |
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